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In Vitro Data

The 3 distinct layers of the 3C Patch®

Fibrin layer

Polymerized fibrin forms during the coagulation step and provides moisture retention and structural integrity for the 3C Patch®.

Platelet layer

In wound healing medical literature it is acknowledged that platelets provide growth factors that are needed to grow blood vessels and form new granulation tissue.32

Leukocyte layer

In wound healing medical literature, it is acknowledged that leukocytes orchestrate healing and fight infection.32 This layer is to be placed directly in contact with the wound.

Patch-histology

Histological stain highlighting the three layers of the 3C Patch®

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Scanning Electron Microscopy image of the 3C Patch®

Growth factors found higher than in standard pure platelet-rich plasma (P-PRP)

Standard P-PRP is pure platelet-rich plasma. P-PRP products consist of platelets without leukocytes in plasma and are used either as a fluid for injection into orthopedic injuries or as a gel for topical application to skin wounds.

The levels of some of the growth factors from 3C Patch® were compared with standard P-PRP3 and the results showed an increase in the concentration of the measured growth factors.

Growth factor PGDF-AB levels were 2.6 times higher than standard P- PRP.4

Growth factor VEGF was 9.8 times higher than standard P-PRP.4

Growth Factors

Growth factors PDGF-AB and TGF-beta.4

n=10, 95% CI

Continuous release has been demonstrated in-vitro

It has been demonstrated, in-vitro, that growth factor PDGF-AB is released continuously from 3C Patch® for at least 1 week and the addition of chronic wound fluid increased the rate of release.4

The 3C Patch® contains a vast amount of growth factors and cytokines, some at very high levels. In vitro analysis has shown that the 3C Patch® responds appropriately to stimuli relevant for wound healing.27

Release of PDGF-AB over time

3C Patch® responses to wound related stimuli in vitro.4

ADAPTIVE NATURE OF 3C PATCH®

More than 800 different growth factors, cytokines and proteins have been detected in the 3C Patch® by multiplex analysis – of these, approximately 380 were differentially expressed in response to stimuli, indicating the adaptive nature of the construct.28 Specifically, proinflammatory (IL-1β and IL-6) are produced only in response to bacterial components (LPS) whereas anti-inflammatory cytokines (IL-1RA and Il-10) are produced under both pro-, and anti-inflammatory circumstances.

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